1. Laboratory Errors: Mistakes in sample handling, labeling, or interpretation can lead to incorrect results. 2. Testing Limitations: Some tests have inherent limitations. For example, the sensitivity and specificity of a test can affect its accuracy. 3. Cross-Reactivity: Certain tests may react with other substances in the body, leading to a false positive. 4. Human Factors: Errors in test administration or reading results can also contribute to false positives.