1. Sterilize the Cup: Before first use and between cycles, sterilize the cup by boiling it in water. 2. Fold and Insert: Fold the cup (C-fold or punch-down fold) and insert it into the vagina. 3. Create a Seal: Rotate the cup to ensure it has fully opened and formed a seal. 4. Wear and Remove: Wear the cup for up to 12 hours, then pinch the base to break the seal and remove it. 5. Empty and Rinse: Empty the fluid, rinse the cup with water, and reinsert it.