Ovarian Stimulation: The woman's ovaries are stimulated using hormonal medications to produce multiple eggs. Egg Retrieval: Eggs are collected from the ovaries using a minor surgical procedure called transvaginal ultrasound aspiration. Sperm Collection: A semen sample is obtained from the male partner or a sperm donor. In cases of azoospermia, sperm may be retrieved directly from the testes using surgical techniques. Micromanipulation: Under a high-powered microscope, an embryologist uses a fine glass needle to inject a single sperm into the cytoplasm of each mature egg. Fertilization and Embryo Culture: The fertilized eggs (now called embryos) are cultured in the lab for several days to monitor their development. Embryo Transfer: One or more healthy embryos are transferred into the woman's uterus to achieve pregnancy.