The nitrazine paper test relies on the principle that different substances exhibit different pH levels. Vaginal secretions typically have a pH range of 4.5 to 5.5, whereas [amniotic fluid]() is more alkaline, with a pH of 7.0 to 7.5. When nitrazine paper is exposed to a fluid sample, it will change color according to the pH level: - Yellow to green indicates an acidic pH (normal vaginal secretions) - Blue indicates an alkaline pH (possible amniotic fluid)