The workflow of a bead-based assay typically involves several steps:
1. Bead Preparation: Microscopic beads are coated with capture antibodies specific to the target analytes. 2. Sample Incubation: The sample is incubated with the beads, allowing the target molecules to bind to the antibodies. 3. Detection: Secondary antibodies conjugated with fluorescent or enzymatic labels are added to bind to the captured analytes. 4. Readout: The beads are analyzed using a flow cytometer or similar instrument, and the fluorescence or enzymatic activity is measured to quantify the analytes.
