The process begins with the collection of a blood sample, which is then subjected to centrifugation to separate platelet-rich plasma (PRP). The PRP is placed in a cuvette within the aggregometer. Various agonists (such as ADP, collagen, or thrombin) are added to the sample to induce platelet aggregation. The change in light transmission is measured and recorded, providing a profile of platelet response to each agonist.