1. Sample Collection: A sample is collected from the affected area, which could be the vagina, cervix, or urethra. This is usually done using a sterile swab. 2. Laboratory Analysis: The sample is cultured in a laboratory where it is placed in a growth medium to encourage the growth of any microorganisms present. 3. Identification: Once growth occurs, the microorganisms are identified through various biochemical tests and sometimes genetic methods.